BD-4, human recombinant | 4525

Defensins are cationic peptides with a large spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The Alpha defensins are differentiated from the Beta-defensins by the pairing of their 3 disulfide bonds. Four human Beta-defensins have been identified to date; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence (for BD-1 a propeptide region). Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta Defensin-4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 50 amino acids. The BD-4 is purified by proprietary chromatographic techniques.

4525 | BD-4 human recombinant DataSheet

Biomolecule/Target: BD-4

Synonyms: DEFB104A; DEFB4; BD-4; DEFB-4; DEFB104; MGC118942; MGC118944; MGC118945; hBD-4

Alternates names: C-C motif chemokine 24, Small-inducible cytokine A24, Myeloid progenitor inhibitory factor 2, CK--6, Eosinophil chemotactic protein 2, Eotaxin-2, CCL24, Ckb-6, MPIF2, MPIF-2, SCYA24, Eotaxin2, CCL-24.

Taglines: An antimicrobial peptide

NCBI Gene ID #: 6369

NCBI Gene Symbol: CCL24

Gene Source: Human

Accession #: O00175

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: 98%

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract using peripheral blood eosinophils using a concentration of 50-100 ng/ml

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 8.8 kDa

Concentration: N/A

Appearance: Lyophilized protein

Physical form description: Sterile filtered and lyophilized from 20 mM PBS, pH 7.4 and 130 mM NaCl.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 µg/µl. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Amino acid sequence: N/A