BHLHA15 Antibody | 7529

BHLHA15 Antibody | 7529 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (71%)

Immunogen: BHLHA15 antibody was raised against a 18 amino acid peptide near the center of human BHLHA15.
The immunogen is located within amino acids 60 - 110 of BHLHA15.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: BHLHA15 antibody can be used for detection of BHLHA15 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: BHLHA15 antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1468 - Rat Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 21 kDa
Observed: 24 kDa

Validation: N/A

Isoform: N/A

Purification: BHLHA15 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: BHLHA15 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: BHLHA15 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: BHLHA15 Antibody: MIST1, BHLHB8, MIST1, Class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, bHLHa15

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: BHLHA15 (basic helix-loop-helix protein 15) , also known as MIST1 (muscle intestine and stomach expression 1) belongs to the bHLH family of transcription factors and plays a role in regulating the transcriptional activity of MYOD1 in muscle cell development as well as serving as a key regulator of acinar cell function (1, 2) . BHLHA15 contains a basic helix-loop-helix (bHLH) domain and is capable of binding to E-box motifs as a homodimer or a heterodimer with E-proteins. It may also negatively regulate bHLH-mediated transcription through a N-terminal repressor domain (3) . It is expressed in mammary epithelial cells and is essential for the regulation of mammary gland development (4) .