BHLHE40 Antibody (N-term) [APR30652G]

BHLHE40 Antibody (N-term) [APR30652G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H, M

Specificity: This BHLHE40 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-51 amino acids from the N-terminal region of human BHLHE40.

Cellular Localisation: Cytoplasm. Nucleus. Note=Predominantly localized in the nucleus (PubMed:11278694) .

Molecular Weight: 45510

Clone: Polyclonal

Gene Name: BHLHE40

Gene ID: 8553

Function: Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed:12397359, PubMed:18411297) . Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop) (PubMed:14672706) . Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2 (PubMed:15193144) . Represses the activity of the circadian transcriptional activator: CLOCK- ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E- box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed:15560782) . Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2 (PubMed:14672706) . Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity (PubMed:19786558) . May be involved in the regulation of chondrocyte differentiation via the cAMP pathway (PubMed:19786558) . Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-ARNTL/BMAL1 complex (PubMed:28797635) . Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system (PubMed:30012868) .

Summary: Tissue Location: Expressed in cartilage, spleen, intestine, lung, and to a lesser extent in heart, brain, liver, muscle and stomach

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Class E basic helix-loop-helix protein 40, bHLHe40, Class B basic helix-loop-helix protein 2, bHLHb2, Differentially expressed in chondrocytes protein 1, DEC1, Enhancer-of-split and hairy-related protein 2, SHARP-2, Stimulated by retinoic acid gene 13 protein, BHLHE40, BHLHB2, DEC1, SHARP2, STRA13