BI | B2798

BI is a derivative of DFHBI (Cat. No. B2797) that binds Broccoli™ with higher affinity and deliver improved cellular fluorescence. In cells, BI has been found to stabilize the Broccoli™ structure and promote its folding. Additionally, Broccoli™/BI complexes is significantly more photostable due to impaired light-induced photoisomerization, and rapid unbinding of photoisomerized cis-BI. BI is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Broccoli™ RNA tag without disrupting biological functions. Importantly, the optimized fluorescence properties of BI enable live single-molecule imaging of Broccoli™-tagged mRNA transcripts in mammalian cells.

BI is a derivative of DFHBI (Cat. No. B2797) that binds Broccoli™ with higher affinity and deliver improved cellular fluorescence. In cells, BI has been found to stabilize the Broccoli™ structure and promote its folding. Additionally, Broccoli™/BI complexes is significantly more photostable due to impaired light-induced photoisomerization, and rapid unbinding of photoisomerized cis-BI. BI is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Broccoli™ RNA tag without disrupting biological functions. Importantly, the optimized fluorescence properties of BI enable live single-molecule imaging of Broccoli™-tagged mRNA transcripts in mammalian cells.

B2798 | BI DataSheet

Alternate Name/Synonyms: (Z)-3-((1H-Benzo[d]imadazol-4-Yl)Methyl)- 5-(3,5-Difluoro-4-Hydroxybenzylidene)-2- Methyl-3,5-Dihydro-4H-Imidazol-4-One

Appearance: Yellow solid

Formulation:

CAS Number:

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₉H₁₄F₂N₄O₂

Molecular Weight: 368.3

Cell-Permeable?: TRUE

Purity: ≥98% by HPLC

Solubilities: >10 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: BI is a DFHBI derivative that displays high Broccoli™ binding affinity and photostability

MDL Number:

PubChem CID: N/A

SMILES: N/A

InChi: N/A

InChi Key: N/A