BID polyclonal Antibody | CAS75116

BID polyclonal Antibody | CAS75116 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Application: WB IP IHC

Application Range: WB: 1:2000~1:5000 IP:1:2000~1:8000 IHC:1:50~1:100

Background: Bid is a pro-apoptotic “BH3 domain-only” member of the Bcl-2 family originally discovered to interact with both the anti-apoptotic family member Bcl-2 and the pro-apoptotic protein Bax. Bid is normally localized in the cytosolic fraction of cells as an inactive precursor and is cleaved at Asp60 by caspase-8 during Fas signaling, leading to translocation of the carboxyl terminal p15 fragment (tBid) to the mitochondrial outer membrane. Translocation of Bid is associated with release of cytochrome c from the mitochondria, leading to complex formation with Apaf-1 and caspase-9 and resulting in caspase-9 activation. Thus, Bid relays an apoptotic signal from the cell surface to the mitochondria triggering caspase activation.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: BID polyclonal Antibody detects endogenous levels of BID protein.

Molecular Weight: ~ 25 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: BH3-interacting domain death agonist; p22 BID; BID; p15 BID; p13 BID; p11 BID

Immunogen: Recombinant protein of human BID.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: