Bim Antibody [1C2H4] | PM-4821

Bim Antibody [1C2H4] | PM-4821 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, ICC, IF

Application: Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 95-107 - Bim Recombinant Protein

Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Bim Monoclonal Antibody is Protein A purified.

Clonality: Monoclonal

Clone: 1C2H4

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Bim Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Bim monoclonal antibody can be stored at -20˚C, stable for one year.

Alternate Name: Bim Antibody [1C2H4] : BAM, BIM, BOD

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.