Bim Antibody | 2065

Bim Antibody | 2065 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-BIM antibody (2065) was raised against a peptide corresponding to 20 amino acids near the amino terminus of human BIM.
The immunogen is located within the first 50 amino acids of BIM.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5 μg/mL; IHC-P: 20 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 4: Cat. No. 1206 - MOLT4 Cell lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22kD
Observed: 22kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BIM expression profile in human and mouse cell lines detected by two independent anti-BIM antibodies that recognize different epitopes, 2065 against N-terminus domain and 3405 against central domain.  BIM proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

KD validation (Figure 9, 11, 12) : Anti-BIM antibody (2065) specificity was further verified by BIM specific knockdown. BIM signal in 293, U937, Jurkat and U266 cells transfected with BIM siRNAs or shRNA was disrupted in comparison with that in cells transfected with control siRNAs or shRNA.

Regulated expression validation (Figure 10) : BIM expression detected by anit-BIM antibodies (2065) was up-regulated by flavopiridol treatment, which was blocked by Cycloheximide in U266 cells.

Isoform: Human BIM has 3 isoforms, including isoform EL (198aa, 22kD) , isoform L (138aa, 16kD) and isoform S (108aa, 13kD) . Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD) , isoform L (140aa, 16kD) and isoform S (110aa, 13kD) . Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD) , isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD) . 2065 can detect all three isoforms.

Purification: Bim Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Bim Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Bim antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Bim Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bim Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bim or BOD in human, mouse and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.