Bim EL Antibody | 7421

Bim EL Antibody | 7421 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Bim EL antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bim.
The immunogen is located within amino acids 20 - 70 of Bim EL.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: Bim EL antibody is human and mouse reactive. Multiple isoforms of Bim are known to exist; this antibody only detects the Bim EL isoform.

Positive Control 1: Cat. No. 1204 - K562 Cell Lysate

Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22 kDa
Observed: 24 kDa

Validation: N/A

Isoform: N/A

Purification: Bim EL Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Bim EL Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Bim EL antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Bim EL Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bim EL Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim, also known as Bcl-2-like protein 11, is a pro-apoptotic member of this family and interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Multiple isoforms of Bim are known to exist, with Bim EL being the longest isoform (1, 2) .