BimS/EL/L Antibody [10B12] | 36-186

BimS/EL/L Antibody [10B12] | 36-186 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Dog, Human, Monkey, Mouse, Rat

Homology: N/A

Immunogen: Synthetic peptide corresponding to aa 20-40 of mouse BimL.

Research Area: Apoptosis, Cancer

Tested Application: E, Flow, ICC, IHC, IP, WB

Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Western Blot: (1:2, 000-1:5, 000 using ECL; suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk; suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa) , BimEL (~23kDa) , and BimS (~16kDa) . Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: 10B12

Isotype: IgG2a, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 0.02% sodium azide and 25% glycerol.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: BCL2L11; Bcl-2-like Protein 11; Bcl2-interacting Mediator of Cell Death

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bim belongs to the Bcl-2 protein family. Bcl-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3) . It has been shown to interact with other members of the Bcl-2 protein family (BCL2, BCL2L1/BCL-X (L) and MCL1) and to act as an apoptotic activator, inducing apoptosis and anoikis. Several variants have been identified. The isoforms vary in cytotoxicity with isoform BimS being the most potent and isoform BimEL being the least potent.