Biotin Antibody [Hyb-8] | 33-236

Biotin Antibody [Hyb-8] | 33-236 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: All species

Homology: N/A

Immunogen: Biotinylated sheep immunoglobulin was used as the immunogen for this Biotin antibody.

Research Area: Other

Tested Application: WB, IHC, Flow, IF, CM

Application: IHC (FFPE) : 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells
IF: 0.5-1 ug/ml
In situ Nucleic Acid Hybridization
WB: 2-5 ug/ml
Confocal Microscopy

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: Hyb-8

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. The mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Biotin antibody may be substituted to decrease this noise.