Biotinylated PD-1/PDCD1, Human Recombinant | P1038

Programmed death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN- secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer. This protein is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. It can also be used as an immunogen, as a protein standard, or in cell biology research applications.

P1038 | Biotinylated PD-1/PDCD1 Human Recombinant DataSheet

Biomolecule/Target: PD1

Synonyms: PDCD1, PD1, CD279, SLEB2, hPD-1, hPD-l

Alternates names: Fatty acid-binding protein heart, H-FABP, Heart-type fatty acid-binding protein, Muscle fatty acid-binding protein, M-FABP, Mammary-derived growth inhibitor, MDGI, FABP3, FABP11, O-FABP.

Taglines: Inhibits the T-cell proliferation and production of IL-1, IL-4, IL-10 and IFN- by suppressing the activation and transduction of PI3K/AKT pathway.

NCBI Gene ID #: 2170

NCBI Gene Symbol: FABP3

Gene Source: Human

Accession #: P05413

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: > 90%

Assay #2: SEC

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its binding ability in functional ELISA

Biological activity: Test in process

Results: Measured by its binding ability to bind Human PD-L1 in a functional ELISA with a linear range of 0.125-1 µg/ml.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 15.1 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 0.5 mg/ml solution in in PBS containing 20% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A