bisBenzimide H 33342 | 2179

A cell-permeable fluorescent DNA stain with low cytotoxicity that binds preferentially to adenine-thymine (A-T) regions of DNA. This stain binds into the minor groove of DNA and exhibits distinct fluorescence emission spectra that are dependent on dye:base pair ratios. May be used for fluorescence microscopy or flow cytometry.

bisBenzimide H 33342 is a a fluorescent DNA stain with low cytotoxicity that binds preferentially to adenine-thymine (A-T) regions of DNA. Useful for staining DNA, chromosomes and nuclei.

2179 | bisBenzimide H 33342 DataSheet

Alternate Name/Synonyms: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

Appearance: Yellow solid

Formulation: N/A

CAS Number: 23491-52-3

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₂₇H₂₈N₆O · 3HCl

Molecular Weight: 561.93

Cell-Permeable?: Yes

Purity: ≥98% by HPLC

Solubilities: H₂O (~ 50 mg/ml)

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A cell-permeable fluorescent DNA stain

MDL Number: MFCD00012678

PubChem CID: 16760503

SMILES: CCOC1=CC=C(C=C1)C2=NC3=C(N2)C=C(C=C3)C4=NC5=C(N4)C=C(C=C5)N6CCN(CC6)C.Cl.Cl.Cl

InChi: InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H

InChi Key: JABNPSKWVNCGMX-UHFFFAOYSA-N