BMP-4 (Human) ELISA Kit | E5087-100

BMP-4 (Human) ELISA Kit | E5087-100

Description: Bone morphogenetic protein 4 (BMP-4) belongs to the transforming growth factor-β (TGF-β) signaling family and has a very broad biological role in development. It plays an essential role in many developmental processes, including neurogenesis, vascular development, angiogenesis and osteogenesis. BMP-4 is highly conserved evolutionarily. It is found in early embryonic development in the ventral marginal zone and in the eye, heart blood and otic vesicle. BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 also plays important roles in adipose tissue: it is essential for white adipogenesis, and promotes adipocyte differentiation. Additionally, it is also important for brown fat, where it induces UCP1, related to non-shivering thermogenesis. ’s BMP-4 (Human) ELISA Kit is used for the quantitative detection of BMP-4 in serum, plasma, tissue genates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of BMP-4 in the sample.

Alternate Name: BMP-4 ELISA Kit; BMP2B ELISA Kit; BMP2B1 ELISA Kit; MCOPS6 ELISA Kit; ZYME ELISA Kit; DVR4 ELISA Kit; OFC11 ELISA Kit; ZYME ELISA Kit; Bone morphogenetic protein 4 ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of BMP-4

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of BMP-4 in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Sensitivity: 18.75 pg/ml Recovery Rate: 85-100% for serum, 88-100% for EDTA plasma and 91-103% for heparin plasma Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Highly sensitive and specific Detection range: 31.25-2000 pg/ml