BMP-5 (Human) ELISA Kit | E5088-100

BMP-5 (Human) ELISA Kit | E5088-100

Description: Bone morphogenetic protein 5 (BMP-5) belongs to the transforming growth factor-β (TGF-β) signaling family and has a very broad biological role in development. It plays an essential role in many developmental processes, including cartilage and bone formation or neurogenesis. BMP-5 act as an important signaling molecule within the trabecular meshwork and optic nerve head, and may play a potential role in glaucoma pathogenesis. It initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2. BMP-5 plays a role in certain cancers. It acts as a key regulator of basal prostate stem cell homeostasis and identifies a potential therapeutic approach against Pten-deficient cancers. BMP5 accelerates expression of hypertrophy markers which is of relevance in both development and diseases such as osteoarthritis. ’s BMP-5 (Human) ELISA Kit is used for the quantitative detection of 5 in serum, plasma, tissue homogenates and other biological fluids. It is based on the principle of sandwich ELISA. The capture antibody is pre-coated on 96-well plates. The standards, test samples and biotin conjugated detection antibody are added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin is added and unbound conjugates are washed away with wash buffer. The HRP enzymatic reaction is detected using TMB-substrate. Finally, an acidic stop solution terminates the enzymatic reaction. The color developed is directly proportional to the amount of BMP-5 in the sample.

Alternate Name: BMP-5 ELISA Kit; Bone Morphogenetic Protein 5 ELISA Kit

Tag Line: A highly sensitive sandwich ELISA kit for the quantitative measurement of BMP-5

Summary: N/A

Detection Method: Absorbance is measured at 450 nm

Sample Type: Human Serum, Plasma, Tissue lysates and other biological fluids

Species Reactivity: Human

Applications: The ELISA kit is used for the quantitative detection of BMP-5 in serum, plasma, tissue homogenates and other biological fluids

Features & Benefits: Sensitivity: 0.094 ng/ml Detection range: 0.156-10 ng/ml Assay Precision; Intra-Assay CV < 8% and Inter-Assay < 10% Recovery Rate: 92-103% for serum, 85-102% for EDTA plasma and 87-103% for heparin plasma Highly sensitive and specific