BMP-9/GDF-2, human recombinant | 7154

GDF-2 belongs to the TGF- cytokine family whose members play an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs. GDF-2 is expressed mainly in non-parenchymal cells of the liver, but is also found in other various cells and tissues. GDF-2 can signal through the ALK1 receptor and has been implicated in a number of physiologic events. These include regulation of the hepatic reticulo-endothelial system, glucose homeostasis, and iron homeostasis, as well as the inhibition of angiogenesis. Recombinant human GDF-2 is a 24.1 kDa disulfide linked homodimeric protein consisting of two 110 amino acid polypeptide chains.

7154 | BMP-9/GDF-2 human recombinant DataSheet

Biomolecule/Target: BMP-9

Synonyms: Growth/Differentiation Factor-2, BMP-9

Alternates names: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF

Taglines: Plays an important role during prenatal development and postnatal growth, remodeling and maintenance of a variety of tissues and organs

NCBI Gene ID #: 3082

NCBI Gene Symbol: HGF

Gene Source: Human

Accession #: P14210

Recombinant: Yes

Source: CHO cells

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.5-1.9 ng/ml.

Biological activity: The ED, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80.017 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18M-cm¹ HO not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: N/A