Bovine Serum Albumin – Heat Shock, Fatty Acid Free, pH 7.0 | 7921

This BSA was purified by a proprietary heat shock process. BSA makes up approximately 60% of all proteins in animal serum. It is commonly used in cell culture protocols, particularly when protein supplementation is necessary and the other components of serum are unwanted. It aids in stabilization of enzymes and other proteins to prevent proteolysis and denaturation, especially when the proteins are present in low concentrations. In cell culture its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger for removing toxic substances, including pyrogens, from the medium.

7921 | Bovine Serum Albumin – Heat Shock, Fatty Acid Free, pH 7.0 DataSheet

Sort Name: Bovine Serum Albumin – Heat Shock, Fatty Acid Free, pH 7.0

Label Name: BSA – HS, Fatty Acid Free, pH 7.0

Taglines: Heat shock precipitated, fatty acid free BSA for multiple applications

Product Highlights: SOURCE: The raw material used to manufacture this lot was derived exclusively from USDA-regulated abattoirs located in the United States. The animals received ante-mortem and post-mortem inspection under a licensed veterinarian’s supervision and were certified at the time of slaughter as free of infectious or contagious diseases and injurious parasites. PURITY: ≥ 96% by SDS-PAGE analysis MOL. WEIGHT: ~ 66.43 kDa FORM: Off-white powder ALBUMIN CONTENT: ≥ 98% by electrophoresis. pH: 7.0 ± 0.5 MOISTURE: ≤ 5.0% HEAVY METALS: ≤ 20 ppm ASH: ≤ 3% FREE FATTY ACIDS: < 0.02% SOLUBILITY: ~ 50 mg/ml in water, with 15 min manual stirring. APPLICATIONS/ADVANTAGES: • Normally used in Serum Free Media as a carrier protein for trace elements, fatty acids, hormones and growth factors. • Allows the formulation of albumin with specific fatty acids to enhance cell culture system performance. • A preferred alternative in assays where the presence of lipids or fatty acids compromises hormone or cholesterol readouts.

Packaging: EA