BPTF bromodomain (2796-2907 aa) (GST-tagged), Human recombinant | 7662

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BPTF is the largest component of the NURF chromatin remodeling complex. It includes adjacent PHD and bromodomains which recognize trimethylation of H3K4 or acetylation of lysines in histone 4, respectively. BPTF is an essential regulator of gene expression in early mouse embryos, and its knock-out is embryonic lethal. Duplication of the BPTF gene has been suggested to increase proliferation of cultured cancer cells, and aberrant BPTF copy numbers were found in 42% of the “NCI-60” panel of 60 human cancer cell lines. This protein product contains the bromodomain of BPTF.

7662 | BPTF bromodomain 2796-2907 aa GST-tagged Human recombinant DataSheet

Biomolecule/Target: BPTF

Synonyms: BPTF bromodomain (2796-2907 aa) (GST-tagged), Human recombinant

Alternates names: Bromodomain PHD Finger Transcription Factor

Taglines: An essential regulator of gene expression

NCBI Gene ID #: 8850

NCBI Gene Symbol: PCAF

Gene Source: Human

Accession #: Q92831

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 40.1 kDa (2796-2907 aa + NT GST Tag)

Concentration: N/A

Appearance: Liquid

Physical form description: 50 mM Tris, pH 7.5, containing 500 mM sodium chloride, 5 mM -mercaptoethanol and 5% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: N/A