BRCA1 Antibody (N-term) [APR04182G]

BRCA1 Antibody (N-term) [APR04182G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This BRCA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 443-472 amino acids from the N-terminal region of human BRCA1.

Cellular Localisation: Nucleus. Chromosome. Cytoplasm. Note=Localizes at sites of DNA damage at double-strand breaks (DSBs) ; recruitment to DNA damage sites is mediated by ABRAXAS1 and the BRCA1-A complex (PubMed:26778126) Translocated to the cytoplasm during UV-induced apoptosis (PubMed:20160719) . [Isoform 5]: Cytoplasm

Molecular Weight: 207721

Clone: Polyclonal

Gene Name: BRCA1

Gene ID: 672

Function: E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage (PubMed:12890688, PubMed:14976165, PubMed:16818604, PubMed:17525340, PubMed:12887909, PubMed:10500182, PubMed:19261748) . It is unclear whether it also mediates the formation of other types of polyubiquitin chains (PubMed:12890688) . The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability (PubMed:12890688, PubMed:14976165, PubMed:20351172) . Regulates centrosomal microtubule nucleation (PubMed:18056443) . Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle (PubMed:10724175, PubMed:12183412, PubMed:11836499, PubMed:19261748) . Required for FANCD2 targeting to sites of DNA damage (PubMed:12887909) . Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation (PubMed:16326698) . Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks (PubMed:19369211) . Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8 (PubMed:16818604) . Acts as a transcriptional activator (PubMed:20160719) .

Summary: Tissue Location: Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:1000

Synonyms: Breast cancer type 1 susceptibility protein, 632-, RING finger protein 53, BRCA1, RNF53