BRD1 bromodomain (556-688 aa) (His-Tag), human recombinant | 7405

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD1 is a bromodomain containing protein that has been identified as a susceptibility gene in neurological disorders, such as schizophrenia and bipolar affective disorder. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. Recombinant human BRD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

7405 | BRD1 bromodomain 556-688 aa His-Tag human recombinant DataSheet

Biomolecule/Target: BRD1

Synonyms: Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD finger-containing protein 2

Alternates names: Bromodomain containing 1, DKFZp686F0325, BR140-like protein, BRL, BRPF1, BRPF2, Bromodomain & PHD finger-containing protein 2

Taglines: Protein controlling chromatin structure and gene expression

NCBI Gene ID #: 8850

NCBI Gene Symbol: PCAF

Gene Source: Human

Accession #: Q92831

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.8 kDa (156 aa, 556-688 aa + His Tag), confirmed by MALDI-TOF.

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSEQVAMEL RLTPLTVLLR SVLDQLQDKD PARIFAQPVS LKEVPDYLDH IKHPMDFATM RKRLEAQGYK NLHEFEEDFD LIIDNCMKYN ARDTVFYRAA VRLRDQGGVV LRQARREVDS IGLEEASGMH LPERPA