BRD2 bromodomain (1-455 aa) (His-Tag), human recombinant | 7406

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease. This protein can be used for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.

7406 | BRD2 bromodomain 1-455 aa His-Tag human recombinant DataSheet

Biomolecule/Target: BRD2

Synonyms: RING3, RNF3, Bromodomain containing 4, D6S113E, FSH, FSRG1, NAT, RING3, RNF3

Alternates names: RING3, RNF3, Bromodomain containing 4, D6S113E, FSH, FSRG1, NAT, RING3, RNF3

Taglines: A protein involved in chromatin targeting

NCBI Gene ID #: 397027

NCBI Gene Symbol: CAPN1

Gene Source: Human

Accession #: P35750

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 85%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 52.8 kDa (478 aa, 1-455 aa + His Tag), confirmed by MALDI-TOF.

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml in Phosphate buffer saline (pH 7.4) containing 10% glycerol and 1 mM DTT.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MGSMLQNVTP HNKLPGEGNA GLLGLGPEAA APGKRIRKPS LLYEGFESPT MASVPALQLT PANPPPPEVS NPKKPGRVTN QLQYLHKVVM KALWKHQFAW PFRQPVDAVK LGLPDYHKII KQPMDMGTIK RRLENNYYWA ASECMQDFNT MFTNCYIYNK PTDDIVLMAQ TLEKIFLQKV ASMPQEEQEL VVTIPKNSHK KGAKLAALQG SVTSAHQVPA VSSVSHTALY TPPPEIPTTV LNIPHPSVIS SPLLKSLHSA GPPLLAVTAA PPAQPLAKKK GVKRKADTTT PTPTAILAPG SPASPPGSLE PKAARLPPMR RESGRPIKPP RKDLPDSQQQ HQSSKKGKLS EQLKHCNGIL KELLSKKHAA YAWPFYKPVD ASALGLHDYH DIIKHPMDLS TVKRKMENRD YRDAQEFAAD VRLMFSNCYK YNPPDHDVVA MARKLQDVFE FRYAKMPD