BRD3-Bromodomain1 (29-145 aa) (His-Tagged) human recombinant | 7256

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD3 binds hyper-acetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. It regulates transcription by promoting the binding of the transcription factor GATA1 to its targets and transcription of the CCND1 gene. A chromosomal aberration involving BRD3 was found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. The recombinant protein includes Bromodomain-containing protein 3 (contains 29-145 aa) with N-terminal His-tag.

7256 | BRD3-Bromodomain1 29-145 aa His-Tagged human recombinant DataSheet

Biomolecule/Target: BRD3

Synonyms: KIAA0043, RING3L

Alternates names: Scatter Factor (SF), Hepatopoietin (HPTA), HGF, HGFB, F-TCF

Taglines: A protein involved in chromatin targeting

NCBI Gene ID #: 3082

NCBI Gene Symbol: HGF

Gene Source: Human

Accession #: P14210

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED, calculated by the dose-dependent proliferation of monkey 4MBr-5 indicator cells was found to be 20-40 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 80.017 kDa

Concentration: 2 mg/ml

Appearance: Liquid

Physical form description: rh-BRD3-BD1 is supplied as a solution in PBS Buffer containing 10% glycerol

Reconstitution Instructions: It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor (HGF) in sterile 18M-cm¹ HO not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: N/A