BRD3-Bromodomain2 (306-417 aa) (His-Tagged) human recombinant | 7257

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. BRD3 binds hyper-acetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. It regulates transcription by promoting the binding of the transcription factor GATA1 to its targets and transcription of the CCND1 gene. A chromosomal aberration involving BRD3 was found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. The recombinant protein includes Bromodomain-containing protein 3 (contains 306-417 aa) with N-terminal His-tag.

7257 | BRD3-Bromodomain2 306-417 aa His-Tagged human recombinant DataSheet

Biomolecule/Target: BRD3

Synonyms: KIAA0043, RING3L

Alternates names: ART, ARTN , EVN, NBN, Enovin, Neublastin

Taglines: A protein involved in chromatin targeting

NCBI Gene ID #: 9048

NCBI Gene Symbol: ARTN

Gene Source: Human

Accession #: NM_057090

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED determined by it's ability to stimulate the proliferation of human SH-SY5Y cells is 2-5 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 24.2 kDa

Concentration: 2 mg/ml

Appearance: Liquid

Physical form description: rh-BRD3-BD2 is supplied as a solution in PBS Buffer containing 10% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-0.5 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A