BRI1 | Brassinosteroid insensitive 1 | AS12 1859

BRI1 | Brassinosteroid insensitive 1 | AS12 1859 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Western blot (WB)

Related Products: AS12 1858 | Anti-BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1, rabbit antibodiesAS16 3203 | Anti-BIN2 | brassinosteroid insensitive 2, rabbit antibodiesAS16 3204 | Anti-SOBIR1 | supressor of BIR1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 5000 (WB)

Molecular weight: above 130 kDa (due to N-glycosylation)

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Brassica napus, Brassica rapaSpecies of your interest not listed? Contact us

Not reactive in: Hordeum vulgare, Oryza sativa, Solanum lycopersicum

Additional Information: Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.

Background: BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G39400

Category: Brassinosteroids/regulation

Research Area: Plant Biology