Bu-1b Antibody [5-11G2] | 99-294

Bu-1b Antibody [5-11G2] | 99-294 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken

Homology: N/A

Immunogen: Bursa cells from two- to five-week-old CHA (Bu-1b) strain chickens

Research Area: Other

Tested Application: Flow

Application: Bu-1b Antibody [5-11G2] for use in flow cytometry, immunohistochemistry / immunocytochemistry, immunoprecipitation, and apoptosis assays.

Specificiy: Bu-1b

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [5-11G2]

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: ChB6.2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Chicken Bu-1b, a product of the Bu-1b allele, is expressed on the cell surface as a disulfide-linked homodimer. In 10-week-old H.B15 and H.B2 chickens, Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. In chickens heterozygous for the Bu-1 alleles (Bu-1a/b) , Bu-1a does not exhibit allelic exclusion. The monoclonal antibody 5-11G2 reacts with lymphoid cells from CHA and H.B14Ab strains but does not react with cells from H.B14Aa