c-Jun Transcription Factor Activity Assay Kit (Colorimetric) | K2097-100

c-Jun Transcription Factor Activity Assay Kit (Colorimetric) | K2097-100

Description: Activator protein-1 (aka activating protein 1, AP-1) is a critical transcription factor that plays a central role in the regulation of genes important in many cellular processes including cell proliferation, differentiation, apoptosis , survial, migration etc. AP-1 family is composed of homodimers and heterodimers of Jun, Fos, ATF or MAF protein families, which are characterized by the highly conserved dimeric basic leucine zipper (bZIP) DNA-binding domains. AP-1 protein is primarily regulated at the level of both Jun and Fos gene transcription involving MAPKs pathways and by post-translational modification via phosphorylation and dephosphorylation. AP-1 activity is induced by either physiological stimuli or environmental insults, including TPA, growth factors, polypeptide hormones, cytokines, UV irradiation, bacterial or viral infections etc. ’s c-Jun Transcription Factor Activity Assais a 96-well plate based colorimetric assay to measure the activation of human or mouse c-Jun in nuclear extracts or cell lysates. The kit offers an easy, rapid, sensitive and non-radioactive way to detect the activation of c-Jun in samples. In this assay, double stranded DNA sequence containing the c-Jun consensus binding site is coated on the 96-well plate. Active c-Jun in the cell lysate or the nuclear extract binds to the oligonucleotides on the plate. After the addition of c-jun primary antibody that recognizes the c-Jun-oligonucleotide complex, a HRP-conjugated secondary antibody is added followed by the addition of TMB substrate and a color signal is developed, which is measured at 450 nm.

Alternate Name: c-Jun Transcription Factor Activity Assay Kit c-Jun Transcription Factor Activity Assay c-Jun Transcription Factor Activity Kit (Colorimetric)

Tag Line: A 96-well plate based colorimetric assay to measure the activation of human or mouse AP-1/c-Jun in nuclear extracts or cell lysates.

Summary: ’s c-Jun Transcription Factor Activity Assay is a 96-well plate based colorimetric assay to measure the activation of human or mouse AP-1/c-Jun in nuclear extracts or cell lysates.

Detection Method: Absorbance at 450 nm

Sample Type: Nuclear extracts Cell lysates

Species Reactivity: Human, Mouse

Applications: Semi-quantitative measurement of activation of mouse or human AP-1/c-Jun in nuclear extracts or cell lysates.

Features & Benefits: The kit offers an easy, rapid, sensitive and non-radioactive way to detect the activation of AP-1/c-Jun in sample