C-Myc Antibody | 6767

c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half-lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastoma. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.

6767 | C-Myc Antibody DataSheet

Antibody Target: C-Myc

Target Alternative Name: MYC; BHLHE39; Myc proto-oncogene protein; Class E basic helix-loop-helix protein 39; Proto-oncogene c-Myc; Transcription factor p64

Tag Line: Polyclonal Antibody to detect c-Myc in human samples. Predicted reactivity with Mouse, Rat, Bovine and Pig samples also.

Category: C-Myc

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human. Predicted reactivity with Mouse, Rat, Bovine and Pig samples.

Immunogen Sequence: This MYC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from human MYC.

Accession #: 4609

Gene ID: P01106

Appearance: Colorless liquid

Form: Liquid

Concentration: N/A

Formulation: Supplied in in PBS with 0.09% (W/V) sodium azide.

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Application: Western blot, FACS.

Positive Control: Western blot: 293 cell lysate, FACS: 293 cells

Application And Usages: Western blot: ~1:1000, FC: 1:10 – 1:50.

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Verified