CAMK1b, Active | 7729

Many of the effects of calcium are mediated via its interaction with calmodulin and the subsequent activation of Ca(2+)/calmodulin-dependent (CaM) kinases. CaM kinases are involved in a wide variety of cellular processes including muscle contraction, neurotransmitter release, cell cycle control, and transcriptional regulation. While CaMKII has been implicated in learning and memory, the biological role of the other multifunctional CaM kinases, CaMKI and CaMKIV, is larGely unknown. CaMKI, or pregnancy upregulated non-ubiquitously expressed CaM kinase (PNCK), is a 38-kDa serine/threonine kinase whose catalytic domain shares 45-70% identity with members of the CaM kinase family. The gene for CaMKI localizes to mouse chromosome X. CaMKI is upregulated during intermediate and late stages of murine fetal development with highest levels of expression in developing brain, bone, and gut. CaMKI is also expressed in a tissue-specific manner in adult mice with highest levels of expression detected in brain, uterus, ovary, and testis. Interestingly, CaMKI expression in these tissues is restricted to particular compartments and appears to be further restricted to subsets of cells within those compartments. The chromosomal localization of CaMKI, along with its tissue-specific and restricted pattern of spatial expression during development, suggests that CaMKI may be involved in a variety of developmental processes including development of the central nervous system (1). Also CaMKI2, an isoform of mCaMKI, was mainly identified in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS, the expression of CaMKI2 is detected in the mantle zone, but not in the ventricular zone, suggesting its possible involvement in the differentiation of neurons (2).

7729 | CAMK1b Active DataSheet

Biomolecule/Target: N/A

Synonyms: CAMK1b, Calcium/calmodulin-dependent protein kinase type I beta chain

Alternates names: CAMK1b, Calcium/calmodulin-dependent protein kinase type I beta chain

Taglines: Involved in many signaling cascades

NCBI Gene ID #: 12370

NCBI Gene Symbol: CASP8

Gene Source: N/A

Accession #: Q3U607

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 223 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 64.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: N/A

Amino acid sequence: N/A