CAMK2A Antibody | 42-002

CAMK2A Antibody | 42-002 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: The immunogen for this antibody is: C-PRTAQSEETRVWHR

Research Area: Signal Transduction

Tested Application: E, WB, IHC, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 58-60kDa band observed in Human (Cerebellum) , Mouse and Rat Brain lysates (calculated MW of 55.3kDa according to Human NP_057065.2, and 54.1kDa according to to Mouse NP_803126.1 and Rat NP_037052.1) . An additional band of 50kDa was observed in some Rat Brain lysates and wss successfully blocked by incubation with the immunizing peptide. The observed molecular weights correspond to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.03--0.3ug/ml. Primary incubation 1 hour at room temperature.Immunohistochemistry:In paraffin embedded Human Brain Cortex shows granular staining in the periphery of pyramydal cell bodies. Recommended concentration: 3-6ug/ml. Paraffin embedded Human Adrenal Gland. Recommended concentration: 3.75ug/ml.Immunofluorescence: Strong expression of the protein seen in the membranes of U2OS and U251 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of Kelly cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognize both reported isoforms (NP_057065.2; NP_741960.1) .

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: CAMK2A, calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, CAMKA, KIAA0968, CaM kinase II alpha subunit, CaM-kinase II alpha chain, CaMK-II alpha subunit, OTTHUMP00000165787, OTTHUMP00000165788, calcium/calmodulin-dependent protein kinase II alpha-B subunit, calcium/calmodulin-dependent protein kinase IIA, calcium/calmodulin-dependent protein kinase type II alpha chain

User Note: N/A

BACKGROUND: N/A