CAMK4, Active | 7740

CAMK4 belongs to the serine/threonine protein kinase family and Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution. Sikela et al. identified that brain CaM kinase IV (CAMK4) appeared to be restricted to brain in the rat; interestingly, it was not detected in the brain of the newborn, but became detectable within a few days after birth. They also raised the possibility that a defect in Camk4 may be responsible for 1 of the mutant phenotypes in the centromeric region near 2 mutations, which known to affect neurologic function and fertility (1). Wei et al. found Camk4-labeled neurons in the hippocampus, amygdala, anterior cingulate cortex, somatosensory cortex, and insular cortex (2). Kang et al. generated transgenic mice in which the expression of a dominant-negative form of Camk4 was restricted to the postnatal forebrain. In these transgenic mice, activity-induced Creb phosphorylation and Fos expression were significantly attenuated. The results indicated that neural activity-dependent CAMK4 signaling in the neuronal nucleus plays an important role in the consolidation/retention of hippocampus-dependent long-term memory (3). Wu et al. generated transgenic mice that selectively express in skeletal muscle a constitutively active form of CAMK4 and concluded that a calcium-regulated signaling pathway controls mitochondrial biogenesis in mammalian cells (4). Wei et al. studied pain and fear memory in Camk4-null mice and concluded that Camk4 is crucial in the trapping of Ca2(+)/calmodulin complexes in neuronal nuclei and in Creb phosphorylation and activation (5).

7740 | CAMK4 Active DataSheet

Biomolecule/Target: N/A

Synonyms: CAMK, Calcium/calmodulin-dependent protein kinase

Alternates names: CAMK, Calcium/calmodulin-dependent protein kinase, CaM kinase-GR

Taglines: Involved in many signaling cascades

NCBI Gene ID #:

NCBI Gene Symbol:

Gene Source: N/A

Accession #:

Recombinant: Yes

Source: Baculovirus (Sf9 insect cells)

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): 83 nmol phosphate incorporated into Autocamtide 2 per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 Ci/assay).

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 79.0 kDa

Concentration: 5µg/50 µl

Appearance: Liquid

Physical form description: Recombinant protein in storage buffer (50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).

Reconstitution Instructions: N/A

Amino acid sequence: N/A