Canine D2D (D-Dimer) ELISA Kit | ELK9371

Canine D2D (D-Dimer) ELISA Kit | ELK9371 | Gentaur UK, US & Europe Distribution

Alternative Names: D 2 Dimer

Species: Canine

Assay Type: Competitive Inhibition

Sensitivity: 27.2 ng/mL

Standard: 5000 ng/mL

Detection Range: 78.13-5000 ng/mL

Sample Type: Plasma

Assay Lenght: 2h

Research Area: Hematology;Cardiovascular biology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with D-Dimer (D2D) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to D-Dimer (D2D) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of D-Dimer (D2D) in the samples is then determined by comparing the OD of the samples to the standard curve.