Carbonic anhydrase II protein | 30R-1325

Carbonic anhydrase II protein | 30R-1325 |

Activity Code: ACTIVE

Product Type: Proteins

Product Subtype: Recombinant Proteins

Research Area: Signal Transduction

Short Description: Purified recombinant Human Carbonic anhydrase II protein

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: Human

Residues: 1-260 amino acids: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Tag/conjugate: N/A

Protein Type: Recombinant

Expression System: E.coli

Grade & Purity: > 95% pure

Methode of Purification: N/A

Source: N/A

Concentration: 1 mg/ml

Form & Buffer: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, containing 1mM DTT, 50mM NaCl, and 10% glycerol.

Storage: Store at 4 deg C for short term storage (1/2 weeks) . Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.

Shipping Information: *Blue Ice*

Applications: SDS-PAGE

Bioactivity: Specific activity is > 7, 000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37 deg C.

Usage Recommendations: N/A

Assay Information: 1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5.2. Dilute 4-Nitrophenyl-Acetate (4-NPA) to 2 mM in Assay Buffer. 3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer. 4. Load 50 uL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 uL of 2 mM Substrate. 5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.