Carbonic Anhydrase XIV/CA14, Human Recombinant | P1582

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA14 is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. Recombinant human CA14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

P1582 | Carbonic Anhydrase XIV/CA14, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: Carbonic anhydrase 14, CAXiV

Alternates names: Carbonic anhydrase 14, CAXiV

Taglines: Zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide.

NCBI Gene ID #: 23632

NCBI Gene Symbol: CA14

Gene Source: Human

Accession #: Q9ULX7

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >85%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): > 700 pmol/min/ug

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit is is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37°C.

Molecular Weight: 33.2 kDa (298aa) (N terminus His-Tag)

Concentration:

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 mM DTT

Reconstitution Instructions:

Amino acid sequence: aa 16-290

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans