Carboxylesterase 1D, mouse recombinant | P1094

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

P1094 | Carboxylesterase 1D mouse recombinant DataSheet

Biomolecule/Target: Ces1d

Synonyms: Ces1d, Carboxylesterase 3, FAEE synthase, TGH, Ces3

Alternates names: Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19

Taglines: Protein involved in the metabolism of xenobiotics and of natural substrates

NCBI Gene ID #: 329244

NCBI Gene Symbol: IL19

Gene Source: Mouse

Accession #: Q8CJ70

Recombinant: Yes

Source: Insect cell (Baculovirus)

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1 EU/g

Activity (Specifications/test method): Measured by the hydrolysis of 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.

Biological activity: Mouse IL-19 is fully biologically active when compared to standard. Significant STAT3 activation is observed with 100 ng/ml of recombinant mouse IL-19.

Results: > 80,000 pmol/min/ug

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to pnitrophenol per minute at pH 7.5 at 37C.

Molecular Weight: 17.7 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-0.5 mg/ml. The solution can be diluted into other aqueous buffers

Amino acid sequence: N/A