This convenient double medium set comprises CARD FERTIUP^® PM and CARD MEDIUM^® that when used together synergize to elevate fertilization rates in mouse IVF. The former is a sperm fertility-enhancing preculture medium. The latter is a novel fertilization-promoting culture medium that acts during IVF on the mouse egg zona pellucida.

CARD FERTIUP^® Preincubation Medium: PM is a preculture medium that enhances mouse sperm fertility. Used in IVF with thawed C57BL/6 mouse sperm frozen using CARD FERTIUP^® Cryoprotectant: CPA, fertilization rates of 30% or more can be expected. CARD FERTIUP^® Preincubation Medium: PM also effectively promotes IVF with fresh sperm.

CARD MEDIUM^® is a novel culture medium that acts during IVF on the mouse egg zona pellucida to promote high fertilization rates, especially when used in conjunction with CARD FERTIUP^® Preincubation Medium: PM. CARD MEDIUM^® was developed by The Center for Animal Resources and Development (CARD), Kumamoto University, Japan.

Combined use of FERTIUP^® sperm cryoprotectant and CARD FERTIUP^® Preincubation Medium: PM greatly improves the fertilization rate of frozen-thawed sperm in C57BL/6 and 129 strains, from less than 10% using conventional methods to more than 30%. Further, when coupled with our new CARD MEDIUM^®, fertility rates are boosted to over 80%. Thus, combined usage of the trio of CARD FERTIUP^® Cryoprotectant: CPA, CARD FERTIUP^® Preincubation Medium: PM and CARD MEDIUM^® provides a high and consistent rate of fertilization from frozen-thawed mouse sperm, which will allow you to efficiently recover mouse lines when needed.