CARD10 Antibody | 3013

CARD10 Antibody | 3013 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10.
The immunogen is located within the last 50 amino acids of CARD10.

Research Area: Apoptosis

Tested Application: E, WB, ICC, IF

Application: CARD10 antibody can be used for detection of CARD10 by Western blot at 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1287 - EL4 Cell Lysate

Positive Control 2: Cat. No. 1288 - A20 Cell Lysate

Positive Control 3: Cat. No. 17-207 - EL4 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 116 kDa
Observed: 115 kDa

Validation: N/A

Isoform: N/A

Purification: CARD10 Antibody is immunoaffinity purified .

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CARD10 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CARD10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: CARD10 Antibody: BIMP1, CARMA3, Caspase recruitment domain-containing protein 10, CARD-containing MAGUK protein 3, Carma 3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CARD10 Antibody: Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD) , death effector domain (DED) , and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex, demonstrating its importance in the regulation of NF-κB transcription factor activation.