CARD9 Antibody (N-term) [AMM22320G]

CARD9 Antibody (N-term) [AMM22320G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This CARD9 antibody is generated from a mouse immunized with a recombinant protein from human CARD9.

Cellular Localisation: Cytoplasm

Molecular Weight: 62241

Clone: Monoclonal

Gene Name: CARD9 {ECO:0000303|PubMed:11053425, ECO:0000312|HGNC:HGNC:16391}

Gene ID: 64170

Function: Adapter protein that plays a key role in innate immune response against fungi by forming signaling complexes downstream of C- type lectin receptors (PubMed:26961233, PubMed:33558980) . CARD9- mediated signals are essential for antifungal immunity against a subset of fungi from the phylum Ascomycota (PubMed:24231284, PubMed:25702837, PubMed:25057046, PubMed:26679537, PubMed:26961233, PubMed:26521038, PubMed:27777981, PubMed:29080677, PubMed:33558980) . Transduces signals in myeloid cells downstream of C-type lectin receptors CLEC7A (dectin- 1), CLEC6A (dectin-2) and CLEC4E (Mincle), which detect pathogen- associated molecular pattern metabolites (PAMPs), such as fungal carbohydrates, and trigger CARD9 activation (By similarity) . Upon activation, CARD9 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to activation of NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways which stimulate expression of genes encoding pro-inflammatory cytokines and chemokines (PubMed:11053425, PubMed:26488816, PubMed:31296852, PubMed:26961233, PubMed:33558980) . CARD9 signaling in antigen-presenting cells links innate sensing of fungi to the activation of adaptive immunity and provides a cytokine milieu that induces the development and subsequent of interleukin 17-producing T helper (Th17) cells (PubMed:24231284) . Also involved in activation of myeloid cells via classical ITAM- associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity) . CARD9 can also be engaged independently of BCL10: forms a complex with RASGRF1 downstream of C-type lectin receptors, which recruits and activates HRAS, leading to ERK activation and the production of cytokines (By similarity) . Acts as an important regulator of the intestinal commensal fungi (mycobiota) component of the gut microbiota (PubMed:33548172) . Plays an essential role in antifungal immunity against dissemination of gut fungi: acts by promoting induction of antifungal IgG antibodies response in CX3CR1 (+) macrophages to confer protection against disseminated C.albicans or C.auris infection (PubMed:33548172) . Also mediates immunity against other pathogens, such as certain bacteria, viruses and parasites; CARD9 signaling is however redundant with other innate immune responses (By similarity) . In response to L.monocytogenes infection, required for the production of inflammatory cytokines activated by intracellular peptidoglycan: acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF- kappa-B (By similarity) .

Summary: Tissue Location: Expression is restricted to several populations of phagocytes, such as macrophages, monocytes, and dendritic cells (PubMed:33548172) . Highly expressed in spleen (PubMed:11053425) . Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain (PubMed:11053425) .

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:500 WB--1:2000

Synonyms: Caspase recruitment domain-containing protein 9, hCARD9, CARD9