Cardif Antibody [Adri-1] | 36-098

Cardif Antibody [Adri-1] | 36-098 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human

Homology: N/A

Immunogen: Recombinant human Cardif (aa 160-450) .

Research Area: Immunology, Innate Immunity

Tested Application: ICC, IHC, IP, WB

Application: Immunocytochemistry: (1:100) . Immunohistochemistry: (paraffin sections) . Immunoprecipitation: (1:200) . Western Blot: (1:1, 000) .

Specificiy: Recognizes human Cardif.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: Adri-1

Isotype: IgG2b

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: CARD Adapter Inducing Interferon-beta; IPS-1; MAVS; Mitochondrial Antiviral Signaling Protein; VISA; Virus-induced Signaling Adapter; Interferon-beta Promoter Stimulator Protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: RIG-I (retinoic acid-inducible gene I; Ddx58) and MDA5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-kappaB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a new CARD (caspase activation and recruitment domain) -containing adaptor protein that interacts with the CARD domain of RIG-I and MDA5, leading to the activation of NF-kappaB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-beta production.