Cardiotrophin-1, Murine Recombinant | 7121

CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which is expressed in various tissues including the adult heart, skeletal muscle, ovary, colon, prostate and fetal lung and signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence. Recombinant murine Cardiotrophin-1 is a 21.3 kDa protein consisting of 202 amino acid residues.

7121 | Cardiotrophin-1 Murine Recombinant DataSheet

Biomolecule/Target: Cardiotrophin-1

Synonyms: CT-1

Alternates names: IL10, D ZCYTO10, IL-20, MGC96907, Four helix cytokine, Zcyto10,

Taglines: A pleiotropic cytokine which is expressed in various tissues

NCBI Gene ID #: 50604

NCBI Gene Symbol: IL20

Gene Source: Mouse

Accession #: Q9NYY1

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: < 0.1 ng/g of protein (<1EU/g).

Activity (Specifications/test method): Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is 1.0 ng/ml, corresponding to a specific activity of 1 x 106 units/mg.

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Sterile filtered through a 0.2 micron filter. Lyophilized from 0.5 mg/ml in 5 mM Tris, pH 8.0

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 µg/µl. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum. This solution can then be stored at 4°C for 1 week or -20°C for future use.

Amino acid sequence: N/A