CASP4 Antibody (C-Term) [APR33189G]

CASP4 Antibody (C-Term) [APR33189G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This CASP4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 337-372 amino acids from human CASP4.

Cellular Localisation: Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion Inflammasome. Secreted Note=Predominantly localizes to the endoplasmic reticulum (ER) Association with the ER membrane requires TMEM214 (PubMed:15123740) Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630) .

Molecular Weight: 43262

Clone: Polyclonal

Gene Name: CASP4 {ECO:0000303|PubMed:15123740, ECO:0000312|HGNC:HGNC:1505}

Gene ID: 837

Function: Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS (PubMed:23516580, PubMed:24879791, PubMed:25119034, PubMed:22246630, PubMed:26174085, PubMed:26173988, PubMed:26508369, PubMed:25964352) . Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1 (PubMed:7797510, PubMed:23516580) . Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators (PubMed:26375003, PubMed:32109412) . Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion (PubMed:25121752, PubMed:26375003, PubMed:25964352, PubMed:32109412) . Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C- terminal (GSDMD-CT) part (PubMed:32109412) . Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation (PubMed:25121752, PubMed:26375003, PubMed:25964352) . Involved in LPS- induced IL6 secretion; this activity may not require caspase enzymatic activity (PubMed:26508369) . Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found Alzheimer's patient brains (PubMed:15123740, PubMed:22246630, PubMed:23661706) . Activated by direct binding to LPS without the need of an upstream sensor (PubMed:25119034) . Does not directly process IL1B (PubMed:7743998, PubMed:7797510, PubMed:7797592) . During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation (PubMed:28314590) .

Summary: Tissue Location: Widely expressed, including in keratinocytes and colonic and small intestinal epithelial cells (at protein level) . Not detected in brain.

Form: N/A

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Caspase-4, CASP-4, ICE (rel) -II, Protease ICH-2, Protease TX, Caspase-4 subunit 1, Caspase-4 subunit 2, CASP4, ICH2