Caspase-1, human recombinant | 1081

Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active human caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200). The rh-Caspase has an N-term His-tag and corresponds to amino acids 120-404 of Caspase-1, gene accession# NP_150634.1.

1081 | Caspase-1 human recombinant DataSheet

Biomolecule/Target: Caspase-1

Synonyms: Caspase-1

Alternates names: IL10, D ZCYTO10, IL-20, MGC96907, Four helix cytokine, Zcyto10,

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 50604

NCBI Gene Symbol: IL20

Gene Source: Human

Accession #: Q9NYY1

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 97%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): > 5000 units/mg

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Semi-Dry powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 g/l. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Amino acid sequence: N/A