Caspase-1, mouse recombinant | 1181

Caspase-1 (also know as ICE) is a prototypical member of the caspase-family of cysteine proteases. Caspase-1 exists in cells as an inactive 45 kDa proenzyme. The pro-enzyme is matured by proteolysis to yield large (20 kD) and small (10 kD) subunits. The active caspase-1 is a heterotetramer consisting of two large and two small subunits. To date the regulatory mechanism of caspase-1 activation and the role of caspase-1 in apoptosis are poorly understood. In THP-1 cells, a large proportion of the caspase-1 is present in the inactive proenzyme form. The recombinant active mouse caspase-1 was expressed in E. coli. The active caspase-1 preferentially cleaves caspase-1 substrates (e.g., YVAD-AFC or YVAD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-YVAD-pNA (Cat. #1104-200) or Ac-YVAD-AFC (1103-200).

1181 | Caspase-1 mouse recombinant DataSheet

Biomolecule/Target: Caspase-1

Synonyms: Caspase 1

Alternates names: IL10, D ZCYTO10, IL-20, MGC96907, Four helix cytokine, Zcyto10,

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 58181

NCBI Gene Symbol: IL21

Gene Source: Mouse

Accession #: Q9JKV9

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): >3500 units/mg

Biological activity: The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with 100 ng/ml of recombinant mouse IL-20.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant caspase-1 is the enzyme activity that cleaves 1 nmol of the caspase substrate Ac-YVAD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Semi-Dry

Physical form description: Semi-Dry

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to mix). The solution can be diluted into other aqueous buffers

Amino acid sequence: N/A