Caspase-10/b, human recombinant | 1090B

Caspase-10b (also known as FLICE 2) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10/b was expressed in E. coli. The active caspase-10 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).

1090B | Caspase-10/b human recombinant DataSheet

Biomolecule/Target: Caspase-10/b

Synonyms: Caspase-10/b

Alternates names: Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 21943

NCBI Gene Symbol: TNF11

Gene Source: Human

Accession #: O35235

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): > 15000 units/mg

Biological activity: Measured by its ability to induce osteoclast formation in RAW264.7 cells using a concentration of 5-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant caspase-10/b is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: 19.4 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg /ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A