Caspase-11 Antibody [8A5] | 36-182

Caspase-11 Antibody [8A5] | 36-182 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse, Rat

Homology: N/A

Immunogen: Recombinant mouse caspase-11 (p20 fragment) .

Research Area: Apoptosis, Cancer, Innate Immunity

Tested Application: E, Flow, IP, WB

Application: ELISA: (recombinant protein) . Flow Cytometry: (overexpressed in cell lines) . Immunoprecipitation: (1-5ug/ml) Western Blot: (1-2ug/ml) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes mouse and rat full-length caspase-11 protein. Detects two bands of ~38kDa and ~43kDa by Western blot.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: 8A5

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: Caspase-11 (mouse) ; Protease ICH-3 (mouse)

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-11 not only activates caspase-1, that is required for the maturation of proinflammatory cytokines such as interleukin-1 (IL-1) and interleukin-18 (IL-18) , but also activates caspase-3, leading to cellular apoptosis under pathological conditions. In most cells, caspase-11 is only expressed upon induction with pro-inflammatory stimuli. Caspase-11 is an essential mediator of endotoxic shock and caspase-11-deficient mice are resistant to endotoxic shock. LPS-induced caspase-11 regulates lymphocyte apoptosis by activating both caspase-3 and caspase-7.