Caspase-12 Antibody [12G6] | 36-184

Caspase-12 Antibody [12G6] | 36-184 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Mouse

Homology: N/A

Immunogen: Recombinant mouse caspase-12 (p20 fragment) and synthetic peptide corresponding to aa 183-205 of mouse caspase-12.

Research Area: Apoptosis, Cancer

Tested Application: E, Flow, WB

Application: ELISA: (direct and indirect: 1:2, 000-1:5, 000) . Immunoprecipitation: (1:500-1:5, 000) . Western Blot: (1:500-1:5, 000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature) . Optimal conditions must be determined individually for each application.

Specificiy: Recognizes an epitope within the p20 fragment of mouse caspase-12. Detects a band of ~51kDa by Western blot. Does not cross-react with mouse caspase-1 or -11.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: >95% (SDS-PAGE)

Clonality: Monoclonal

Clone: 12G6

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Liquid. In PBS containing 0.02% sodium azide.

Concentration: 1 mg/ml

Storage Condition: Stable for at least 1 year after receipt when stored at -20˚C.

Alternate Name: CASP-12

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-12 is a member of the ICE/CED-3 family of caspases and is detected as a protein of ~51kDa. Overexpression of caspase-12 in cells induces apoptosis. Capase-12 is localized to the endoplasmatic reticulum (ER) and activated by ER stress and specifically cleaves and activates pro-caspase-9 and is also thought to be involved in the apoptosis induced by viral infection. Caspase-12-deficient mice are resistant to ER stress-induced apoptosis. Caspase-12 is probably expressed in many and perhaps all mouse tissues and is expressed at high levels in skeletal muscle, heart, lung and kidney and at moderate levels in brain and liver. In many humans caspase-12 is a pseudo gene and not expressed.