Caspase-12 Antibody | 2327

Caspase-12 Antibody | 2327 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Caspase-12 antibody was raised against a 17 amino acid peptide near the center of murine Caspase-12.
The immunogen is located within amino acids 90 - 140 of Caspase-12.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: Caspase-12 antibody can be used for detection of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 3: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 4: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 55 kDa

Validation: N/A

Isoform: N/A

Purification: Caspase-12 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Caspase-12 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-12 Antibody: Caspase-12, CASP-12

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-12 Antibody: Three distinct signaling pathways lead to programmed cell death (apoptosis) . The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP) . Caspase-12 mediates cytotoxicity induced by amyloid-beta.