Caspase-2 Antibody | 3447

Caspase-2 Antibody | 3447 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (94%) , Chicken: (75%)

Immunogen: Caspase-2 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-2.
The immunogen is located within the first 50 amino acids of Caspase-2.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, ICC

Application: Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: Depending on cell lines or tissues used, other cleavage products may be observed.

Positive Control 1: Cat. No. 1314 - Human Thymus Tissue Lysate

Positive Control 2: Cat. No. 1305 - Human Kidney Tissue Lysate

Positive Control 3: Cat. No. 1288 - A20 Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 51 kDa
Observed: 50 kDa

Validation: N/A

Isoform: N/A

Purification: Caspase-2 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Caspase-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-2 Antibody: ICH1, NEDD2, CASP-2, NEDD-2, PPP1R57, ICH1, Caspase-2, Neural precursor cell expressed developmentally down-regulated protein 2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-2 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis. Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.