Caspase 3 Antibody, KO Validated | 18-541

Caspase 3 Antibody, KO Validated | 18-541 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 55-160 of human Caspase 3 (NP_004337.2) .

Research Area: Apoptosis, Cell Cycle, Neuroscience, Signal Transduction

Tested Application: WB, IHC

Application: WB: 1:500 - 1:2000IHC: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: Jurkat

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Observed: 17kDa, 35kDa

Validation: Antibody is Knockout validated.

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.