Caspase-3, human recombinant | 1083

Caspase-3 (also know as CPP32, Yama and apopain) is a major member of the caspase-family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. During apoptosis procaspase-3 is processed at aspartate residues by self-proteolysis and/or cleavage by upstream caspases, such as caspase-6 (Mch2), caspase-8 (Flice) and grazyme B. The processed form of caspase-3 consists of large (17 kD) and small (11 kD) subunits which associate to form the active enzyme. The active caspase-3 has been shown involving in the proteolysis of several important molecules, such as poly (ADP-ribose) polymerase (PARP), the sterol regulatory element binding proteins (SREBPs), focal adhesion kinase (FAK), and others. The recombinant active human caspase-3 expressed in E. coli spontaneously undergoes autoprocessing to yield subunits characteristic of the native enzyme (Full length gene Accession No. NP_004337) . The active caspase-3 preferentially cleaves caspase-3 substrates (e.g., DEVD-AFC or DEVD-pNA) and is routinely tested at for its ability to enzymatically cleave these two substrates Ac-DEVD-pNA (Cat. #1008-200) or Ac-DEVD-AFC (1007-200).

1083 | Caspase-3 human recombinant DataSheet

Biomolecule/Target: Caspase-3

Synonyms: Caspase-3

Alternates names: Soluble Receptor Activator of NFkB Ligand, TNFSF11, TRANCE, TNF-related activation-induced cytokine, OPGL, ODF, Osteoclast differentiation factor, Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa B ligand, RANKL, Osteoprotegerin ligand, CD254 antigen, sRANKL, sOdf.

Taglines: A cysteine-aspartic acid protease involved in apoptosis

NCBI Gene ID #: 21943

NCBI Gene Symbol: TNF11

Gene Source: Human

Accession #: O35235

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Measured by its ability to induce osteoclast formation in RAW264.7 cells using a concentration of 5-10 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.4 kDa

Concentration: N/A

Appearance: Semi-Dry

Physical form description: Semi-Dry

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg /ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A