Caspase-3 (Pro and Active) Antibody [CAS3836] | 33-007

Caspase-3 (Pro and Active) Antibody [CAS3836] | 33-007 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: The large subunit domain of human Caspase-3 was used as the immunogen for this monoclonal antibody.

Research Area: Apoptosis

Tested Application: WB

Application: Western blot: 2-4 ug/ml
This antibody will detect the pro form and p17 and p12 active forms of Caspase-3.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G purified monoclonal antibody

Clonality: Monoclonal

Clone: CAS3836

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.5 mg/mL

Storage Condition: Aliquot and Store at -20˚C. Avoid freez-thaw cycles.

Alternate Name: Caspase-3 Antibody: CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: Caspases, or cysteine-aspartic proteases, are a family of cysteine proteases that play essential roles in inflammation, apoptosis and necrosis. They are often referred to as the 'executioners of apoptosis.' Caspases are synthesized as inactive proenzymes comprising an N-terminal peptide (prodomain) together with one large and one small subunit. Active caspase-1 and -3 are heterodimers, containing two small and two large subunits. Active Caspase-3 subunits are 17kD (p17) and 12kD (p12) . Pro Caspase-3 is inactive and has virtually no activity until it is cleaved by an initiator caspase. It is activated by Caspases -8, -9 and -10 ('initiator caspases') and then effects apoptosis by cleaving targeted cellular proteins.