Caspase-3 Substrate DEVD-pNA | 1008

Ready-to-use colorimetric substrate for CPP32/caspase-3 and related caspases that recognize the amino acid sequence DEVD. The sequence DEVD is based on caspase-3 cleavage site in poly (ADP-ribose) polymerase (PARP). CPP32 and related caspase activity can be quantified by spectrophotometric detection of free pNA (l = 400 nm) after cleavage from the peptide substrate DEVD-pNA, using a spectrophotometer or multi-well plate reader. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amounts of caspase assays.

Caspase-3 Substrate DEVD-pNA is a ready-to-use fluorometric substrate for caspase-3/CPP32 (Km = 9.7 µM) and related caspases that recognize the amino acid sequence DEVD.

1008 | Caspase-3 Substrate DEVD-pNA DataSheet

Alternate Name/Synonyms: Ac-DEVD-pNA, Caspase-3 Substrate, Colorimetric

Appearance: Liquid

Formulation: In DMSO ( 4 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Asp-Glu-Val-Asp-pNA

Salt Form: No

Molecular Formula: C₂₆H₃₄N₆O₁₃

Molecular Weight: 638.58

Cell-Permeable?: No

Purity: >98% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: Caspase-3 substrate

MDL Number: MFCD00792707

PubChem CID: 4329

SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C

InChi: InChI=1S/C26H34N6O13/c1-12(2)22(26(43)30-18(11-21(38)39)24(41)28-14-4-6-15(7-5-14)32(44)45)31-23(40)16(8-9-19(34)35)29-25(42)17(10-20(36)37)27-13(3)33/h4-7,12,16-18,22H,8-11H2,1-3H3,(H,27,33)(H,28,41)(H,29,42)(H,30,43)(H,31,40)(H,34,35)(H,36,37)(H,38,39)

InChi Key: GGXRLUDNGFFUKI-UHFFFAOYSA-N